The Biological Imaging Facility (BIF, formerly called the Biological Imaging Center, BIC) is the only core facility on the Caltech campus providing light microscopy resources.
The BIF has 5 confocal microscopes for optical sectioning of fluorescently labeled samples. Four are Zeiss laser scanning confocal microscopes (LSMs) and one is a Perkin-Elmer Ultra View Spinning Disc confocal on a Zeiss Microscope which is our sole high speed confocal microscope. Three of these confocal microscopes have multiphoton/two-photon lasers (MP) which allow for deeper imaging of tissues. Two have non-descanned detectors (NDDs) for great sensitivity. The sixth microscope is a Leica DMI 6000 wide field fluorescence microscope for deconvolution analyses. The Leica and three of the confocal microscopes are equipped with incubation chambers for controlling temperature and/or CO2 levels.
In addition to microscopes, the BIF also provides software resources for data processing. The BIF has two computer workstations running the Imaris image processing software from Bitplane. A third computer workstation is running the Huygens software from Scientific Volume Imaging B.V. (SVI).