STATUS UPDATES: Spinning Disc temporarily unavailable while LSM 880 is being moved into its room.
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Welcome to the

Biological Imaging Facility

    The Biological Imaging Facility (BIF, formerly called the Biological Imaging Center, BIC) is the only core facility on the Caltech campus providing light microscopy resources.

    The BIF has 5 confocal microscopes for optical sectioning of fluorescently labeled samples. Four are Zeiss laser scanning confocal microscopes (LSMs) and one is a Perkin-Elmer Ultra View Spinning Disc confocal on a Zeiss Microscope which is our sole high speed confocal microscope. Three of these confocal microscopes have multiphoton/two-photon lasers (MP) which allow for deeper imaging of tissues. Two have non-descanned detectors (NDDs) for great sensitivity. The sixth microscope is a Leica DMI 6000 wide field fluorescence microscope for deconvolution analyses. The Leica and three of the confocal microscopes are equipped with incubation chambers for controlling temperature and/or CO2 levels.

    In addition to microscopes, the BIF also provides software resources for data processing. The BIF has two computer workstations running the Imaris image processing software from Bitplane. A third computer workstation is running the Huygens software from Scientific Volume Imaging B.V. (SVI).

Microscopes

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  • Zeiss LSM 710 (Rm 33A)
  • Perkin-Elmer Spinning Disc confocal (Rm 33D)
  • Zeiss LSM 880 (Rm 33B)
  • Leica DMI 6000 (Rm 33F)
  • Zeiss LSM 510 META (Rm 33G)
  • Zeiss LSM 800 (Rm 33H)
  • Documentation for all the microscopes and software is provided at the following link.

Contact

Director of the BIF:

Andres Collazo, Ph.D

  • Office: 626-395-2761
  • BIF: 626-395-2863
  • acollazo@caltech.edu