4D Imaging of the Embryonic Zebrafish Heart Development
Movie: Michael Liebling and Arian Forouhar

This 80 hpf Tg(gata1:GFP) zebrafish, counterstain with Bodipy dye (in gray), was imaged on an LSM 5 LIVE confocal microscope (Carl Zeiss GmbH) and rendered with Imaris software (Bitplane AG).

Further reading:

Liebling, M., Forouhar, A.S., Gharib, M., Fraser, S.E. and Dickinson, M.E. (2005)
"4-Dimensional Cardiac Imaging in Living Embryos via Post-Acquisition Synchronization of Nongated Slice-Sequences".
Journal of Biomedical Optics, 10(5): 054001-1 – 054001-10.

Forouhar, A.S., Liebling, M., Hickerson, A., Nasiraei-Moghaddam, A., Tsai, H.J., Hove, J.R., Fraser, S.E., Dickinson, M.E., and Gharib, M. (2006)
"The embryonic vertebrate heart tube is a dynamic suction pump".
Science 312: 751-753

Liebling, M., Forouhar, A.S., Wolleschensky, R., Zimmermann, B., Ankerhold, R., Fraser, S.E., Gharib, M., Dickinson, M.E. (2006)
"Rapid Three-Dimensional Imaging and Analysis of the Beating Embryonic Heart Reveals Functional Changes During Development".
Developmental Dynamics 235(11): 2940-2948.